anti prkn  (Cell Signaling Technology Inc)

Journal: Cell & Bioscience

Article Title: PINK1 functions in mitophagy and mitochondrial homeostasis during mice oocyte maturation

doi: 10.1186/s13578-025-01460-4

Figure Lengend Snippet: RAB8A is required for PINK1-mediated mitophagy in murine oocytes. ( A ) The effects of Pink1 -OE on PRKN and RAB8A protein levels, as detected by Western blot. Proteins from 300 oocytes were loaded for every sample. ( B ) Relative intensity results for the protein expression of PRKN in control and Pink1 -OE oocytes. ( C ) Relative intensity results for the protein expression of RAB8A in control and Pink1 -OE oocytes. ( D ) The effects of 10 μm CCCP on PINK1, PRKN, and RAB8A protein levels, as detected by Western blot. ( E ) Relative intensity results for the protein expression of PINK1 in control and 10 μm CCCP treated oocytes. ( F ) Relative intensity results for the protein expression of PRKN in control and 10 μm CCCP treated oocytes. ( G ) Relative intensity results for the protein expression of RAB8A in control and 10 μm CCCP treated oocytes. Proteins from 300 oocytes were loaded for every sample

Article Snippet: Rabbit monoclonal anti-γH2AX antibody (Cat#: ab81299), rabbit monoclonal anti-RAB8A antibody (Cat#: ab188574), mouse monoclonal anti-Myc antibody (Cat#: ab18185) were purchased from Abcam (Cambridge, UK); rabbit polyclonal anti-PINK1 antibody (Cat#: PA5-85243) was obtained from Thermo Fisher Scientific (Thermo Fisher Scientific, USA); mouse monoclonal- FITC-conjugated anti-α-Tubulin antibody (Cat#: F2168) was bought from Sigma (St. Louis, MO, USA); rabbit polyclonal antibodies anti-LC3 antibody (Cat#: 14600-1-AP), mouse-Monoclonal antibody (Cat#: 60004-1-Ig), rabbit polyclonal antibody anti-α-Tubulin antibody (Cat#: 11224-1-AP) were purchased from Proteintech (Wuhan, China); mouse monoclonal antibody anti-PRKN antibody (Cat#: sc-32282) was obtained from Santa Cruz Biotechnologies (Santa Cruz, CA).

Techniques: Western Blot, Expressing, Control

Journal: Cell & Bioscience

Article Title: PINK1 functions in mitophagy and mitochondrial homeostasis during mice oocyte maturation

doi: 10.1186/s13578-025-01460-4

Figure Lengend Snippet: RAB8A activity is essential for murine oocyte quality in advanced maternal age. (A) The effects of advanced maternal age on RAB8A protein level. (B) The effects of advanced maternal age on PRKN protein level. (C) Western blotting with anti-Myc showed that Rab8a - T22N-Myc and Rab8a - Q67L-Myc were efficiently overexpressed. (D) Ultrastructure observation of Young, Old, Old + Rab8a - WT, Old + Rab8a - T22N, Old + Rab8a - Q67L oocytes. Mitophagosomes (red arrows), healthy mitochondria (white arrows), damaged mitochondria (blue arrows). (E) Number of mitophagosomes per cell section in Young, Old, Old + Rab8a – WT, Old + Rab8a – T22N, Old + Rab8a – Q67L oocytes (1.07 ± 0.21; 1.13 ± 0.17; 1.23 ± 0.34; 1.10 ± 0.16; 2.13 ± 0.21, respectively ). Bar graphs are mean ± SD of results from 3 oocytes section. Bars: 500 nm. (The bars labeled with completely different letters indicate significant difference at P < 0.05). (F) A proposed model showing the potential pathway that RAB8A activity is involved in PINK1-PRKN mediated mitophagy in oocytes

Article Snippet: Rabbit monoclonal anti-γH2AX antibody (Cat#: ab81299), rabbit monoclonal anti-RAB8A antibody (Cat#: ab188574), mouse monoclonal anti-Myc antibody (Cat#: ab18185) were purchased from Abcam (Cambridge, UK); rabbit polyclonal anti-PINK1 antibody (Cat#: PA5-85243) was obtained from Thermo Fisher Scientific (Thermo Fisher Scientific, USA); mouse monoclonal- FITC-conjugated anti-α-Tubulin antibody (Cat#: F2168) was bought from Sigma (St. Louis, MO, USA); rabbit polyclonal antibodies anti-LC3 antibody (Cat#: 14600-1-AP), mouse-Monoclonal antibody (Cat#: 60004-1-Ig), rabbit polyclonal antibody anti-α-Tubulin antibody (Cat#: 11224-1-AP) were purchased from Proteintech (Wuhan, China); mouse monoclonal antibody anti-PRKN antibody (Cat#: sc-32282) was obtained from Santa Cruz Biotechnologies (Santa Cruz, CA).

Techniques: Activity Assay, Western Blot, Labeling